7CVK

Crystal structure of glucose isomerase by fixed-target serial synchrotron crystallography (100 ms)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-04-03
Detector	PSI PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	I 2 2 2
Unit cell lengths	94.440, 100.240, 103.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.010 - 1.700
R-factor	0.1815
Rwork	0.181
R-free	0.19940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y4i
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.460	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	54250	5361
<I/σ(I)>	3.36	1.66
Completeness [%]	100.0
Redundancy	241.2
CC(1/2)	0.925	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	293.5	Tris-HCl, Ammonium sulfate, Magnesium sulfate