7CVJ

Crystal structure of lysozyme by fixed-target serial synchrotron crystallography (100 ms)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-04-03
Detector	PSI PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	P 43 21 2
Unit cell lengths	79.450, 79.450, 38.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.180 - 1.500
R-factor	0.1684
Rwork	0.167
R-free	0.18530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jxq
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	20320	1969
<I/σ(I)>	7.94	2.24
Completeness [%]	100.0
Redundancy	427
CC(1/2)	0.997	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293.5	sodium acetate, PEG 8000, NaCl