7CV7
RNA methyltransferase METTL4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.931, 93.719, 96.365 |
| Unit cell angles | 90.00, 104.38, 90.00 |
Refinement procedure
| Resolution | 29.625 - 2.300 |
| R-factor | 0.2216 |
| Rwork | 0.220 |
| R-free | 0.25090 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (7.0.076) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.063 | 0.039 | 0.597 |
| Rmeas | 0.074 | 0.046 | 0.711 |
| Rpim | 0.039 | 0.024 | 0.382 |
| Total number of observations | 159846 | ||
| Number of reflections | 46526 | 4816 | 4278 |
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 98.1 | 99.2 | 91.1 |
| Redundancy | 3.4 | 3.7 | 3.1 |
| CC(1/2) | 0.997 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M sodium malonate pH 5.5 and 6% PEG3350 |
