7CV7

RNA methyltransferase METTL4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97916
Spacegroup name	P 1 21 1
Unit cell lengths	61.931, 93.719, 96.365
Unit cell angles	90.00, 104.38, 90.00

Refinement procedure

Resolution	29.625 - 2.300
R-factor	0.2216
Rwork	0.220
R-free	0.25090
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (7.0.076)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.063	0.039	0.597
Rmeas	0.074	0.046	0.711
Rpim	0.039	0.024	0.382
Total number of observations	159846
Number of reflections	46526	4816	4278
<I/σ(I)>	11.5
Completeness [%]	98.1	99.2	91.1
Redundancy	3.4	3.7	3.1
CC(1/2)		0.997	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M sodium malonate pH 5.5 and 6% PEG3350