7CV6

RNA methyltransferase METTL4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97852
Spacegroup name	I 2 2 2
Unit cell lengths	93.821, 130.022, 141.322
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.010
R-factor	0.2431
Rwork	0.240
R-free	0.30010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5il2
RMSD bond length	0.011
RMSD bond angle	1.595
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.110
High resolution limit [Å]	3.000	6.440	3.000
Rmerge	0.121	0.049	0.400
Rmeas	0.132	0.051	0.482
Rpim	0.049	0.015	0.261
Total number of observations	83220
Number of reflections	14468	1704	938
<I/σ(I)>	6.3
Completeness [%]	82.5	91.6	54.7
Redundancy	5.8	10.7	2.4
CC(1/2)		0.999	0.008

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.49 M NaH2PO4 and 0.91 M K2HPO4,