7CUX
Crystal structure of human Schlafen 5 N'-terminal domain (SLFN5-N) involved in ssRNA cleaving and DNA binding
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 87.15 |
| Detector technology | PIXEL |
| Collection date | 2019-06-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 211.860, 156.442, 131.459 |
| Unit cell angles | 90.00, 110.38, 90.00 |
Refinement procedure
| Resolution | 41.076 - 3.295 |
| R-factor | 0.203555713424 |
| Rwork | 0.201 |
| R-free | 0.24715 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yd0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.759 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1-2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.610 |
| High resolution limit [Å] | 3.294 | 3.300 |
| Number of reflections | 43847 | 2193 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 90.2 | |
| Redundancy | 6.8 | |
| CC(1/2) | 1.000 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277.15 | 100 mM Tris, pH 7.5, 80 mM magnesium chloride, 1.6 M ammonium sulfate |
