7CUI
Crystal structure of fission yeast Pot1 and Tpz1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 126.227, 126.227, 121.355 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.780 - 2.600 |
| R-factor | 0.1977 |
| Rwork | 0.196 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.029 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.093 | 0.043 | 0.660 |
| Rmeas | 0.101 | 0.047 | 0.729 |
| Rpim | 0.040 | 0.019 | 0.303 |
| Total number of observations | 196899 | ||
| Number of reflections | 30605 | 3295 | 2986 |
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 99.7 | 99.7 | 98.7 |
| Redundancy | 6.4 | 6.1 | 5.5 |
| CC(1/2) | 0.998 | 0.759 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 277 | 25% PEG8000, 100 mM sodium citrate and 100 mM Tris-HCl |
| 1 | EVAPORATION | 8 | 277 | 25% PEG8000, 100 mM sodium citrate and 100 mM Tris-HCl |
