7CUG

Crystal Structure of Urate Oxidase from Bacillus sp. TB-90 in the absence from Chloride Anion at 1.62 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-22
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.0000
Spacegroup name	I 2 2 2
Unit cell lengths	71.657, 133.715, 145.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.610 - 1.620
R-factor	0.2207
Rwork	0.219
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wlv
RMSD bond length	0.013
RMSD bond angle	0.989
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.720
High resolution limit [Å]	1.620	1.620
Rmerge	0.138	0.372
Rmeas	0.372	0.413
Number of reflections	87767	2227
<I/σ(I)>	8.1	2
Completeness [%]	98.4	91.9
Redundancy	12.9
CC(1/2)	0.993	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	293.15	16%(w/v) PEG 8000, 0.1M Tris/H2SO4, pH 8.3, 0.09 M K2SO4, 2mM 8-azaxanthine