7CTQ
Peptidyl tryptophan dihydroxylase QhpG essential for tryptophylquinone cofactor biogenesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.610, 51.560, 101.790 |
| Unit cell angles | 90.00, 99.77, 90.00 |
Refinement procedure
| Resolution | 37.758 - 1.978 |
| R-factor | 0.1837 |
| Rwork | 0.182 |
| R-free | 0.22160 |
| Structure solution method | SIRAS |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.758 | 2.100 |
| High resolution limit [Å] | 1.978 | 1.978 |
| Rmerge | 0.058 | 0.530 |
| Rmeas | 0.068 | 0.625 |
| Number of reflections | 63162 | 19439 |
| <I/σ(I)> | 12.7 | 2.22 |
| Completeness [%] | 98.7 | 97.2 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.998 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277.2 | 100 mM HEPES (pH 7.0), 20 mM MgCl2, 17% (v/v) Poly(acrylic acid sodium salt) 5100, 1.8% (w/v) 1,6-hexanediol, 34 mM Cyclohexyl-methyl-beta-D-maltoside |
