7CT8
Crystal structure of apo CmoB from Vibrio Vulnificus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.121, 89.896, 88.711 |
| Unit cell angles | 90.00, 114.76, 90.00 |
Refinement procedure
| Resolution | 48.420 - 2.100 |
| R-factor | 0.1811 |
| Rwork | 0.179 |
| R-free | 0.21330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qnx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.522 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.110 | 0.062 | 1.054 |
| Rmeas | 0.119 | 0.068 | 1.148 |
| Rpim | 0.046 | 0.026 | 0.446 |
| Total number of observations | 370749 | ||
| Number of reflections | 56139 | 2881 | 2769 |
| <I/σ(I)> | 5 | ||
| Completeness [%] | 99.0 | 98.5 | 98.7 |
| Redundancy | 6.6 | 6.7 | 6.1 |
| CC(1/2) | 0.997 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium acetate trihydrate, Sodium formate |
