7CT6
Crystal structure of GCL from Deinococcus metallilatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-04 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979415 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.055, 106.469, 89.093 |
| Unit cell angles | 90.00, 124.09, 90.00 |
Refinement procedure
| Resolution | 46.504 - 2.100 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pan |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.917 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.086 | 0.035 | 0.666 |
| Rmeas | 0.094 | 0.038 | 0.728 |
| Rpim | 0.036 | 0.014 | 0.289 |
| Total number of observations | 435191 | ||
| Number of reflections | 66471 | 3374 | 3282 |
| <I/σ(I)> | 5.1 | ||
| Completeness [%] | 98.7 | 97.8 | 98.4 |
| Redundancy | 6.5 | 6.7 | 6 |
| CC(1/2) | 0.999 | 0.888 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | (18) % (w/v) PEG 400 0.1 M Sodium citrate tribasic pH5.6 0.1 M Sodium chloride |
