7CT1

Crystal structure of Snx27 FERM domain in complex with a DLF motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	43.194, 72.941, 103.999
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.276 - 1.947
R-factor	0.1741
Rwork	0.172
R-free	0.22220
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.720	2.050
High resolution limit [Å]	1.947	1.950
Number of reflections	24036	3158
<I/σ(I)>	23.8
Completeness [%]	96.8
Redundancy	10.7
CC(1/2)	0.999	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.09 M NPS 0.1 M Buffer System 3 pH8.5 50 % v/v Precipitant Mix 4