7CSZ

Crystal structure of the N-terminal tandem RRM domains of RBM45 in complex with single-stranded DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 43
Unit cell lengths	86.546, 86.546, 27.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.637 - 1.800
R-factor	0.1899
Rwork	0.185
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7csx
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.091	0.058	1.391
Rmeas	0.098	0.063	1.496
Rpim	0.036	0.024	0.544
Total number of observations	141140
Number of reflections	19410	2045	1909
<I/σ(I)>	6.4
Completeness [%]	100.0	99.9	100
Redundancy	7.3	6.9	7.4
CC(1/2)		0.997	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M sodium formate, 20% PEG 3350