7CSX

Crystal structure of the N-terminal tandem RRM domains of RBM45

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 43
Unit cell lengths	62.485, 62.485, 52.023
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.184 - 2.500
R-factor	0.2067
Rwork	0.201
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jvx
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.160	0.063	0.950
Rmeas	0.169	0.067	1.009
Rpim	0.052	0.021	0.331
Number of reflections	7056	735	697
<I/σ(I)>	5.6
Completeness [%]	99.7	99.6	99.3
Redundancy	10.9	10.5	9.2
CC(1/2)		0.998	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M Ammonium phosphate dibasic, 20% PEG 3350