7CSR

Structure of Ephexin4 R676L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97876
Spacegroup name	C 2 2 21
Unit cell lengths	147.075, 244.729, 141.744
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.770 - 3.000
R-factor	0.2142
Rwork	0.212
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cso
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	3.000	8.130	3.000
Rmerge	0.147	0.058	1.015
Rmeas	0.160	0.063	1.101
Rpim	0.062	0.025	0.421
Total number of observations	339343
Number of reflections	51159	2717	2528
<I/σ(I)>	3.2
Completeness [%]	99.9	99.7	100
Redundancy	6.6	6	6.7
CC(1/2)		0.996	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M HEPES pH7.5, 0.8 M NAH2PO4, 0.8 M K2HPO4