7CSP

Structure of Ephexin4 IDPSH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97915
Spacegroup name	P 65
Unit cell lengths	143.082, 143.082, 138.543
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.830 - 3.000
R-factor	0.1877
Rwork	0.185
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cso
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.830	46.830	3.190
High resolution limit [Å]	3.000	8.870	3.000
Rmerge	0.089	0.042	0.955
Rmeas	0.104	0.048	1.123
Total number of observations	221044
Number of reflections	59938	2312	9465
<I/σ(I)>	10.35	28.05	1.27
Completeness [%]	94.8	94.2	92.8
Redundancy	3.688	3.628	3.531
CC(1/2)	0.997	0.996	0.476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	2.8 M Sodium acetate, pH 7.0