7CSO

Structure of Ephexin4 DH-PH-SH3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97775
Spacegroup name	P 31 1 2
Unit cell lengths	144.444, 144.444, 290.518
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.420 - 2.390
R-factor	0.1771
Rwork	0.176
R-free	0.20410
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.420	48.420	2.540
High resolution limit [Å]	2.390	7.110	2.390
Rmerge	0.179	0.138	0.769
Rmeas	0.193	0.149	0.830
Total number of observations	1862101
Number of reflections	267055	10182	42812
<I/σ(I)>	6.95	13.43	2.1
Completeness [%]	99.8	99.7	98.7
Redundancy	6.973	6.775	7.047
CC(1/2)	0.987	0.985	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	800 mM Potassium phosphate dibasic, 800 mM Sodium phosphate monobasic, 100 mM HEPES, pH7.5