7CSL

Crystal structure of the archaeal EF1A-EF1B complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2013-11-24
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	55.149, 70.194, 77.822
Unit cell angles	112.90, 91.77, 89.98

Refinement procedure

Resolution	44.410 - 2.000
R-factor	0.2273
Rwork	0.225
R-free	0.27520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wy9 2yy3
RMSD bond length	0.013
RMSD bond angle	1.689
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.410	2.120
High resolution limit [Å]	2.000	2.000
Rmerge	0.046	0.320
Number of reflections	70518	10918
<I/σ(I)>	16.2
Completeness [%]	96.6
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES-NaOH pH 7.0, 10% w/v PEG6000, 5% v/v MPD