7CS9
AtPrR1 in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-12-18 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.727, 134.916, 142.046 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.271 - 2.801 |
| R-factor | 0.224245118234 |
| Rwork | 0.221 |
| R-free | 0.28039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qyd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.293 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.271 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.152 | 1.283 |
| Number of reflections | 39296 | 3860 |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M lithium chloride, 20% w/v PEG 3350 |
