7CS2
Apo structure of dimeric IiPLR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.758, 242.458, 77.562 |
| Unit cell angles | 90.00, 110.53, 90.00 |
Refinement procedure
| Resolution | 31.398 - 2.688 |
| R-factor | 0.201731584446 |
| Rwork | 0.201 |
| R-free | 0.24155 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qyd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.156 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.399 | 2.780 |
| High resolution limit [Å] | 2.688 | 2.690 |
| Rmerge | 0.093 | 0.764 |
| Number of reflections | 70443 | 6742 |
| <I/σ(I)> | 19.8 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 0.2M sodium citrate tribasic, 0.1 M sodium citrate/citric acid, pH 4.0, 20% PEG 3350 |
