7CS0
Aminoglycoside 2'-N-acetyltransferase from Mycolicibacterium smegmatis-Complex with Coenzyme A and Paromomycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-11-06 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97935 |
Spacegroup name | P 32 |
Unit cell lengths | 56.374, 56.374, 122.866 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.200 - 2.050 |
R-factor | 0.18564 |
Rwork | 0.183 |
R-free | 0.23323 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7crm |
RMSD bond length | 0.009 |
RMSD bond angle | 1.643 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.101 | 0.266 |
Number of reflections | 27416 | 1389 |
<I/σ(I)> | 31.56 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.6 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2M calcium acetate hydrate, 0.1M HEPES (pH 7.5), 10% (w/v) PEG 8000 |