7CRV

Crystal structure of rNLRP1-FIIND

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	83.955, 83.955, 156.544
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.760 - 2.000
R-factor	0.2263
Rwork	0.225
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g5b
RMSD bond length	0.011
RMSD bond angle	1.386
Data scaling software	HKL-3000
Phasing software	PHENIX (1.18.2-3874)
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	43870	4107
<I/σ(I)>	30.9
Completeness [%]	99.9
Redundancy	19.3
CC(1/2)	0.997	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.0M Ammonium sulfate, 0.1M Bis-Tris pH 5.5, 1% w/v polyethylene glycol 3350