Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 |
| Unit cell lengths | 185.401, 185.401, 382.849 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.560 - 3.700 |
| R-factor | 0.2959 |
| Rwork | 0.295 |
| R-free | 0.34090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cr6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.949 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.920 |
| High resolution limit [Å] | 3.690 | 3.690 |
| Number of reflections | 157305 | 25305 |
| <I/σ(I)> | 12.68 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.5 | |
| CC(1/2) | 0.998 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% MPD, 0.1 M imidazole pH 6.5, 0.2 M (NH4)2SO4, 10% (w/v) PEG 3350 |
