7CQP

Complex of TRPC4 and Calmodulin_Nlobe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9789
Spacegroup name	P 61 2 2
Unit cell lengths	64.460, 64.460, 120.751
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.988 - 1.900
R-factor	0.221
Rwork	0.219
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1up5
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3228)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.102	0.057	1.004
Rmeas	0.110	0.061	1.076
Rpim	0.039	0.022	0.386
Total number of observations	330733
Number of reflections	12754	2247	2188
<I/σ(I)>	5.6
Completeness [%]	99.7	99.6	99.5
Redundancy	7.4	7.3	7.5
CC(1/2)		0.997	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	55.7% w/v Polyethylene glycol 1000, 150mM MES, 2% v/v 2-Methyl-2,4-pentanediol, pH 6.0