7CQ3
Crystal structure of Slx1-Slx4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.300, 118.421, 62.002 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.821 - 1.449 |
| R-factor | 0.1243 |
| Rwork | 0.123 |
| R-free | 0.15680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cq2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.500 |
| High resolution limit [Å] | 1.449 | 3.120 | 1.450 |
| Rmerge | 0.060 | 0.032 | 0.502 |
| Number of reflections | 92349 | 9510 | 9131 |
| <I/σ(I)> | 11.1 | ||
| Completeness [%] | 99.8 | 98.1 | 100 |
| Redundancy | 5.6 | 5.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2 M ammonium sulfate, 0.1 M Tris 8.5 and 20% PEG 3350 |
