7CQ0

Crystal structure of Streptoavidin-C1 from Streptomyces cinamonensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-08
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9794
Spacegroup name	P 42 21 2
Unit cell lengths	58.442, 58.442, 78.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.880 - 2.030
R-factor	0.2054
Rwork	0.203
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hds
RMSD bond length	0.004
RMSD bond angle	0.621
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.880	2.090
High resolution limit [Å]	2.030	2.030
Rmeas	0.315	0.315
Number of reflections	16769	16769
<I/σ(I)>	6.3
Completeness [%]	99.8
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		298.15	PEG3350