7CP2
Crystal structure of the African swine fever virus core shell protein p15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2019-11-10 |
| Detector | SDMS |
| Wavelength(s) | 1.03836 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.037, 53.601, 74.892 |
| Unit cell angles | 90.00, 92.89, 90.00 |
Refinement procedure
| Resolution | 46.790 - 2.190 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.24610 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.461 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.836 | |
| Rmeas | 0.093 | |
| Number of reflections | 25170 | 2482 |
| <I/σ(I)> | 21.02 | |
| Completeness [%] | 98.8 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M magnesium chloride, 0.1 M Tris-HCl, 20% (w/v) PEG 6000 |
