7COW

353 bp di-nucleosome harboring cohesive DNA termini with linker histone H1.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98.15
Detector technology	PIXEL
Collection date	2019-08-26
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	66.215, 105.053, 171.126
Unit cell angles	86.59, 88.95, 88.25

Refinement procedure

Resolution	49.350 - 2.860
R-factor	0.2264
Rwork	0.225
R-free	0.29710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ut9 4qlc
RMSD bond length	0.006
RMSD bond angle	1.445
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	2.910
High resolution limit [Å]	2.860	2.860
Rmerge	0.091	1.521
Rmeas	0.108	1.858
Rpim	0.057	1.046
Number of reflections	103522	4391
<I/σ(I)>	7.7	0.8
Completeness [%]	97.4	82.9
Redundancy	3.4	2.8
CC(1/2)	0.996	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291.15	potassium acetate, calcium chloride, potassium chloride