7COV

Potato D-enzyme, native (substrate free)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-26
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	69.869, 120.535, 174.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.360 - 2.000
Rwork	0.150
R-free	0.18780
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.803
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.360	2.110
High resolution limit [Å]	2.000	2.000
Rmeas	0.032	0.092
Number of reflections	49562	7039
<I/σ(I)>	44.2
Completeness [%]	99.2
Redundancy	7.3
CC(1/2)	1.000	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		273	12% PEG 8000, 100 mM HEPES (pH 7.6), 100 mM CaCl2