7COU

Structure of cyanobacterial photosystem II in the dark S1 state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	293
Detector technology	CCD
Collection date	2017-04-17
Detector	MPCCD
Wavelength(s)	1.77
Spacegroup name	P 21 21 21
Unit cell lengths	126.150, 232.700, 288.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.965 - 2.250
R-factor	0.1707
Rwork	0.169
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ws5
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.330
High resolution limit [Å]	2.250	2.250
Number of reflections	399661	39640
<I/σ(I)>	65.3
Completeness [%]	100.0
Redundancy	846.8
CC(1/2)	0.999	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	PEG, magnesium sulfate