7COT
Structure of post fusion core of SARS-CoV-2 S2 subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-05-31 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.99984 |
Spacegroup name | P 3 |
Unit cell lengths | 51.968, 51.968, 116.225 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.360 - 2.160 |
R-factor | 0.2017 |
Rwork | 0.197 |
R-free | 0.24660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wyy |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.200 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.088 | 0.510 |
Number of reflections | 18634 | 890 |
<I/σ(I)> | 13.55 | |
Completeness [%] | 98.4 | |
Redundancy | 2.6 | |
CC(1/2) | 0.840 | 0.568 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 25% PEG 3350, 0.07M Bis-Tris propane pH 8.5, 0.03M citric acid |