7COQ
Hexameric Ring Complex of Engineered V1-ATPase bound to AMP-PNP: A3(De)3_(ANP)1cat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-30 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 124.445, 124.486, 131.339 |
| Unit cell angles | 90.00, 93.03, 90.00 |
Refinement procedure
| Resolution | 46.340 - 3.440 |
| R-factor | 0.2142 |
| Rwork | 0.213 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7COP |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18_3845)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.340 | 3.550 |
| High resolution limit [Å] | 3.440 | 3.440 |
| Rmerge | 0.117 | 0.845 |
| Number of reflections | 53244 | 4602 |
| <I/σ(I)> | 11.3 | 2.4 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-HCl (pH 8.5), 22% PEG 3350 and 0.4 M Ammonium Acetate |
