7COA

Ternary complex of DNA polymerase Mu with 1-nt gapped DNA (T:dGMPNPP) and Mn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2019-10-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	59.950, 68.570, 110.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.935 - 1.698
R-factor	0.1762
Rwork	0.175
R-free	0.19910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.935	28.935	1.740
High resolution limit [Å]	1.698	7.590	1.700
Rmerge	0.059	0.034	0.379
Rmeas	0.065	0.039	0.429
Number of reflections	50943	630	3605
<I/σ(I)>	17.3	31.93	3.87
Completeness [%]	99.5	96.2	96.8
Redundancy	5.445	4.673	4.503
CC(1/2)	0.998	0.997	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	298	TRIS-HCL, PEG 3350