7CO6

Binary complex of DNA polymerase Mu with 1-nt gapped DNA (templating thymine)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2018-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	60.180, 62.590, 117.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.448 - 1.900
R-factor	0.1813
Rwork	0.180
R-free	0.21440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.448	29.448	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.049	0.025	0.534
Rmeas	0.055	0.028	0.587
Total number of observations	194141
Number of reflections	35607	448	2580
<I/σ(I)>	17.91	38.79	3.51
Completeness [%]	99.4	95.3	99.8
Redundancy	5.452	4.969	5.662
CC(1/2)	0.999	0.999	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	298	PEG 4000, 2-Propanol, Sodium citrate tribasic dihydrate