7CNX
Crystal structure of Apo PSD from E. coli (2.63 A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-04-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.896, 101.847, 170.095 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.780 - 2.630 |
| R-factor | 0.2338 |
| Rwork | 0.231 |
| R-free | 0.28840 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.430 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.830 | 48.780 | 2.730 |
| High resolution limit [Å] | 2.630 | 9.840 | 2.630 |
| Rmerge | 0.146 | 0.030 | 2.609 |
| Rmeas | 0.156 | 0.032 | 2.783 |
| Rpim | 0.055 | 0.013 | 0.964 |
| Total number of observations | 5526 | 35503 | |
| Number of reflections | 42052 | 895 | 4328 |
| <I/σ(I)> | 12.3 | 50.7 | 1 |
| Completeness [%] | 100.0 | 98.2 | 100 |
| Redundancy | 8 | 6.2 | 8.2 |
| CC(1/2) | 0.998 | 0.999 | 0.441 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.18 M Ammonium acetate, 0.09 M Tris pH 8.5, 22.5% w/v PEG 3350, 5% v/v Jeffamine M-600 pH 7.0 |
