7CNW
Crystal structure of Apo PSD from E. coli (1.90 A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-29 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.443, 79.817, 147.089 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.810 - 1.900 |
| R-factor | 0.2131 |
| Rwork | 0.211 |
| R-free | 0.24330 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.579 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.030 | 49.030 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.137 | 0.035 | 2.802 |
| Rmeas | 0.142 | 0.036 | 2.933 |
| Rpim | 0.037 | 0.011 | 0.845 |
| Total number of observations | 1009308 | ||
| Number of reflections | 71625 | 712 | 3952 |
| <I/σ(I)> | 14.6 | 62 | 1 |
| Completeness [%] | 98.8 | 94.4 | 86.8 |
| Redundancy | 14.1 | 10.6 | 11.4 |
| CC(1/2) | 0.999 | 0.999 | 0.369 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | 0.1 M BIS-TRIS pH 6.3, 28% w/v PEGMME 2000, 5% w/v 1.6-Hexanediol |
