7CNG
Structure of CDK5R1 bound FEM1B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-20 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 126.812, 126.812, 144.489 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.900 - 3.490 |
R-factor | 0.2251 |
Rwork | 0.220 |
R-free | 0.26720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lbf |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 |
High resolution limit [Å] | 3.490 | 3.490 |
Rmerge | 0.047 | 2.271 |
Number of reflections | 15530 | 1510 |
<I/σ(I)> | 24.5 | |
Completeness [%] | 100.0 | |
Redundancy | 25.5 | |
CC(1/2) | 1.000 | 0.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.6M Magnesium sulfate hydrate, 0.1M BIS-TRIS propane pH 6.7 |