7CNB

Crystal structure of Gp16 C-terminal domain from Bacillus virus phi29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 41 2 2
Unit cell lengths	42.682, 42.682, 135.856
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.680 - 2.320
R-factor	0.2495
Rwork	0.249
R-free	0.27080
Structure solution method	SAD
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.680	2.440
High resolution limit [Å]	2.320	2.320
Rmerge	0.121	1.380
Rmeas	0.126	1.434
Number of reflections	6004	835
<I/σ(I)>	17.8	2.9
Completeness [%]	100.0	100
Redundancy	23.3
CC(1/2)	0.999	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.1 M Bis-Tris pH 6.5, 1.8 M ammonium sulfate, 2% PEG monoethyl ether 550