7CNA
Crystal structure of Spindlin1/C11orf84 complex bound to histone H3K4me3K9me3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.603, 101.184, 56.615 |
| Unit cell angles | 90.00, 91.41, 90.00 |
Refinement procedure
| Resolution | 35.110 - 1.600 |
| R-factor | 0.1727 |
| Rwork | 0.172 |
| R-free | 0.20070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mzf |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.798 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.110 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.026 | 0.110 |
| Number of reflections | 66759 | 10727 |
| <I/σ(I)> | 32.7 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M sodium cacodylate pH 6.5, 35% PEG3350, 5% glycerol, 2% Benzamidine hydrochloride |
