7CN7
T4 phage spackle protein gp61.3 complex with lysozyme domain of gp5 tail lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.720, 69.180, 83.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.920 - 1.150 |
| R-factor | 0.1081 |
| Rwork | 0.107 |
| R-free | 0.12150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k28 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.066 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.720 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.044 | 0.542 |
| Rmeas | 0.047 | 0.590 |
| Rpim | 0.018 | 0.230 |
| Number of reflections | 187608 | 4655 |
| <I/σ(I)> | 24.5 | 0.859 |
| Completeness [%] | 99.8 | 97.6 |
| Redundancy | 7.1 | 6.3 |
| CC(1/2) | 1.000 | 0.859 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293.15 | PEG550MME, MES, KSCN |
