7CLT

Crystal structure of the EFhd1/Swiprosin-2, a mitochondrial actin-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	31.770, 47.556, 87.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.599 - 2.074
R-factor	0.209698872726
Rwork	0.209
R-free	0.22894
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i2l
RMSD bond length	0.007
RMSD bond angle	0.888
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.070	2.070
Rmerge	0.046	0.590
Number of reflections	8434	403
<I/σ(I)>	11.1
Completeness [%]	99.2
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100 mM HEPES-NaOH, 5 mM ZnSO4, 25% (v/v) Jeffamine ED-2001