7CLL
Mycobacterium tubeculosis enolase in complex with 2-Phosphoglycerate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9775 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.697, 197.317, 200.553 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.380 - 1.990 |
| R-factor | 0.17716 |
| Rwork | 0.175 |
| R-free | 0.22051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j04 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.603 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 121176 | 5330 |
| <I/σ(I)> | 10.17 | |
| Completeness [%] | 91.8 | |
| Redundancy | 9.1 | |
| CC(1/2) | 0.985 | 0.598 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 20% PEG 3350, 0.2 M ammonium acetate, pH 6.5 Bis-Tris buffer |
