7CKF

The N-terminus of interferon-inducible antiviral protein-dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97879
Spacegroup name	P 21 21 21
Unit cell lengths	65.326, 79.329, 140.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.790 - 2.284
R-factor	0.2144
Rwork	0.212
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b92
RMSD bond length	0.007
RMSD bond angle	1.039
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.280	6.240	2.280
Rmerge	0.221	0.071
Rmeas	0.219	0.074
Rpim	0.087	0.021	0.781
Number of reflections	33591	1845	1654
<I/σ(I)>	2.1
Completeness [%]	100.0	99.9	100
Redundancy	12.7	12	12.6
CC(1/2)		0.999	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		286	sodium formate, PEG 3350