7CK2

Crystal structure of Arabidopsis CESA3 catalytic domain with UDP-Glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9735
Spacegroup name	P 1 21 1
Unit cell lengths	54.360, 148.530, 94.090
Unit cell angles	90.00, 103.15, 90.00

Refinement procedure

Resolution	43.780 - 2.050
R-factor	0.2147
Rwork	0.214
R-free	0.23110
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.817
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.050	2.050
Number of reflections	89995	6579
<I/σ(I)>	12.98	0.99
Completeness [%]	99.0
Redundancy	14.1
CC(1/2)	0.990	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	PEG3350