7CK1
Crystal structure of arabidopsis CESA3 catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-09-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.620, 147.030, 92.510 |
| Unit cell angles | 90.00, 102.05, 90.00 |
Refinement procedure
| Resolution | 48.080 - 2.350 |
| R-factor | 0.2388 |
| Rwork | 0.237 |
| R-free | 0.26390 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.515 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 59250 | 4379 |
| <I/σ(I)> | 8.14 | 0.83 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 11.41 | 12.09 |
| CC(1/2) | 0.990 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | PEG3350 |
