7CJR
Crystal structure of a periplasmic sensor domain of histidine kinase VbrK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 52.550, 52.550, 157.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.680 - 2.280 |
| R-factor | 0.2339 |
| Rwork | 0.232 |
| R-free | 0.27310 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | SHELX |
| Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.680 | 2.881 | 2.340 |
| High resolution limit [Å] | 2.280 | 2.782 | 2.280 |
| Rmerge | 0.051 | 1.860 | |
| Rmeas | 0.052 | 1.898 | |
| Rpim | 0.010 | 0.373 | |
| Number of reflections | 10752 | 589 | 731 |
| <I/σ(I)> | 34.1 | 6 | |
| Completeness [%] | 99.7 | 98.99 | 95.8 |
| Redundancy | 25.1 | 243.6 | 24.3 |
| CC(1/2) | 1.000 | 1.000 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2M NaCl, 25% PEG 3350, 0.1M Bis-Tris pH5.5 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2M NaCl, 25% PEG 3350, 0.1M Bis-Tris pH5.5 |
