7CJN

grass carp interleukin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-17
Detector	AGILENT EOS CCD
Wavelength(s)	0.97923
Spacegroup name	P 65 2 2
Unit cell lengths	83.090, 83.090, 302.931
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.420 - 2.660
R-factor	0.3779
Rwork	0.378
R-free	0.41230
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.175
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.726
High resolution limit [Å]	2.660	2.660
Rmerge	0.200
Number of reflections	17766	16914
<I/σ(I)>	15.3
Completeness [%]	93.1
Redundancy	5.2
CC(1/2)		0.100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	PH8.2, 0.056M Sodium phosphate monobasic monohydrate, 1.344M Potassium phosphate dibasic