7CIZ

Crystal structure of DNAJC9 HBD helix2 in complex with H3.3-H4 dimer and MCM2 HBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97736
Spacegroup name	C 2 2 21
Unit cell lengths	47.638, 176.816, 202.925
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.884 - 1.800
R-factor	0.1727
Rwork	0.171
R-free	0.21380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bnv
RMSD bond length	0.006
RMSD bond angle	0.792
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.073
Rpim	0.024
Number of reflections	78310	3667
<I/σ(I)>	31.3	2.2
Completeness [%]	97.7
Redundancy	10
CC(1/2)		0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.05 M Lithium sulfate, 0.05 M Sodium sulfate; 0.05 M Tris, pH 8.5, 30% (v/v) PEG400.