7CIT

Crystal structure of tyrosinase from Streptomyces castaneoglobisporus in complex with the caddie protein obtained by soaking in the solution containing Cu(II) and hydroxylamine for 24 h

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-17
Detector	RAYONIX MX-225
Wavelength(s)	0.90000
Spacegroup name	P 21 21 2
Unit cell lengths	64.560, 96.080, 54.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.500
R-factor	0.1438
Rwork	0.144
R-free	0.20670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zmy
RMSD bond length	0.031
RMSD bond angle	0.027
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	SHELX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.049	0.432
Number of reflections	53168	5762
<I/σ(I)>	35.6
Completeness [%]	96.3
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	PEG 3350, NaNO3