7CIQ
Phosphorylation modification of MHC I polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.402, 52.352, 93.205 |
| Unit cell angles | 90.00, 93.23, 90.00 |
Refinement procedure
| Resolution | 33.380 - 1.590 |
| R-factor | 0.1736 |
| Rwork | 0.172 |
| R-free | 0.20930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bst |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.859 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.590 | 1.600 |
| Rmerge | 0.079 | 0.413 |
| Number of reflections | 203885 | 203885 |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.2MSodiumchloride,0.1MBis-Tris(pH6.5),25%(w/v)PEG 3,350 |
