7CIK
Structure of the 58-213 fragment of FliF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 121.750, 121.750, 71.721 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.880 - 2.290 |
| R-factor | 0.2155 |
| Rwork | 0.213 |
| R-free | 0.26140 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.082 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.900 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.054 | 0.305 |
| Number of reflections | 17374 | 1750 |
| <I/σ(I)> | 9.8 | 2.9 |
| Completeness [%] | 96.6 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277 | 0.1M Na-K phosphate pH 6.2, 2.5M NaCl |
